Pseudomonas tolaasii is a bacterium which affects the mushroom and springs the most common infection as bacterial blotch disease. It commonly occurs in summer-autumn time; the affected mushroom caps are covered with yellow-brown spots. In severe forms of infection these spots blowout the mushroom and become distorted, putrefied with obnoxious odour. Young pins affected by bacterial blotch disease, become brown and further growth is very rare. The scope and purpose of the study is to acquainted with the efficacy of Homoeopathic medicines in providing resistance to button mushrooms against bacterial blotch disease which infected by Pseudomonas tolaasii and to recognize among the selection of two medicines which was more efficient in providing resistance against bacterial blotch disease. Aconite Napellus was initiate to be effective and categorize the bacterial blotch disease which infected by Pseudomonas tolaasii. It bared amended and enhanced growth of mushroom bud for about five mm against Pseudomonas tolaasii. The approach of achievement for Aconite Napellus was more effective than Belladonna in handling bacterial blotch disease. Thus, Homoeopathic medicines plays a major role in rectifying the fungal infections caused by various pathogens and it promotes the farmer’s economic development by mushroom cultivation in numerous aspects of prosperous progressions.